Deep tissue imaging with multiphoton fluorescence microscopy

Imaging carious dental tissues with multiphoton fluorescence lifetime imaging microscopy

In this study, multiphoton excitation was utilized to image normal and carious dental tissues noninvasively. Unique structures in dental tissues were identified using the available multimodality (second harmonic, autofluorescence, and fluorescence lifetime analysis) without labeling. The collagen in dentin exhibits a strong second harmonic response. Both dentin and enamel emit strong autofluore...

Multiphoton fluorescence microscopy.

Multiphoton fluorescence microscopy has now become a relatively common tool among biophysicists and biologists. The intrinsic sectioning achievable by multiphoton excitation provides a simple means to excite a small volume inside cells and tissues. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal c...

Multiphoton Fluorescence Microscopy

The human body is a constant flux of thousands of chemical/biological interactions and processes connecting molecules, cells, organs, and fluids, throughout the brain, body, and nervous system. Up until recently it was thought that all these interactions operated in a linear sequence, passing on information much like a runner passing the baton to the next runner. However, the latest findings in...

Brain Imaging with Multiphoton Microscopy

Optimizing 3D multiphoton fluorescence microscopy.

We present a new optimization concept for 3D multiphoton fluorescence microscopy by finding the optimal excitation beam giving rise to the smallest possible light-emitting volume or the highest possible signal to noise ratio (SNR).